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NOTE: After the coupling of the primary C-terminal amino acid of all peptides to the amino group of the membrane, the amino teams between the spots and also among the amino teams within the spot areas don't kind bonds with the amino acids. Be sure all of the purification buffers are at four °C and perform the protein purification within the cold room to avoid protein denaturation. NOTICE: This avoids the coupling of amino acids within the additional cycles with the membrane instead of the rising peptide chain.
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